一、测定目的·····················································2
二、黄连及其制剂中生物碱成分简介 ································2
（一）黄连及其主要化学成分·······································2
（二）生物碱成分介绍·············································2
（三）黄连及其制剂中生物碱成分介绍·······························2
（四）黄连及其生物碱类成分的药理作用·····························3
二、HPLC测定方法简述············································4
三、测定方法实例·················································4
1，江斌，姚曦，吉国辉何伟等采用HPLC同时测定黄连提取物中表小檗碱的
含量·····························································4
2，阳勇、朱晶王欣王智民测定黄连药材及其炮制品中盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的含量·································4
3，武小赟李铁钢阳勇钟国跃范昕建张艺罗维等测试黄连主要生物碱含量的测定方法·····························································4
四、讨论总结·····················································5
参考文献·························································5
一、测定目的
建立黄连提取物高效液相色谱法（HPLC）含量测定方法。测定黄连及制剂中盐酸药根碱、盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的含量,。
二、黄连及其制剂中生物碱成分简介。
（一）黄连及其主要化学成分 ：
黄连：又名：味连、川连、鸡爪连。属毛茛科、黄连属多年生草本植物，叶全部基生，叶片坚纸质，卵状三角形，边缘有锐锯齿，侧生裂片不等，叶柄长5-12cm。黄连喜冷凉、湿润、荫蔽，需要温度低、空气湿度大的自然环境，忌高温、干旱。常野生或栽培于海拔1000-1900m的山谷凉湿荫蔽密林中。其味入口极苦，性寒，归心、胃、肝、大肠经，具清热泻火，燥湿，解毒之功效。临床上多用于治疗痢疾、急性胃肠炎、慢性腹泻、呼吸道感染以及各种炎症，近年研究发现其在抗肿瘤、抗心率失常、降血糖等方面亦有明显作用
黄连化学成分有生物碱和木脂素2类。生物碱以小檗碱含量最高，还含黄连碱、甲基黄连碱、巴马亭、药根碱、表小檗碱，掌叶防已碱及木兰花碱等。酸性成分有阿魏酸，氯原酸等, 此外还包括有黄柏酮、黄柏内酯，挥发油、黄酮类、香豆素、萜类、甾体、多糖等成分。
